mouse ccl2/mcp-1 elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: Frontiers in Immunology

Article Title: An efficient functional screening method for anti ATP hydrolase antibody based on fluorescent probes

doi: 10.3389/fimmu.2026.1906242

Figure Lengend Snippet: Antibody function screening flowchart. (A) The structure of adenosine triphosphate. (B) Biochemical system screening process. Created with BioGDP.com . (C) Cell verification system process. Created with BioGDP.com .

Article Snippet: CellCounting-Lite 2.0 Luminescent Cell Viability Assay (CCL2.0) (Vazyme, DD1101).

Techniques: Screening

Journal: Frontiers in Immunology

Article Title: An efficient functional screening method for anti ATP hydrolase antibody based on fluorescent probes

doi: 10.3389/fimmu.2026.1906242

Figure Lengend Snippet: Reagent dosages for biochemical system.

Article Snippet: CellCounting-Lite 2.0 Luminescent Cell Viability Assay (CCL2.0) (Vazyme, DD1101).

Techniques: Positive Control, Negative Control, Solvent

Journal: Frontiers in Immunology

Article Title: An efficient functional screening method for anti ATP hydrolase antibody based on fluorescent probes

doi: 10.3389/fimmu.2026.1906242

Figure Lengend Snippet: Reaction system in the assay plate.

Article Snippet: CellCounting-Lite 2.0 Luminescent Cell Viability Assay (CCL2.0) (Vazyme, DD1101).

Techniques:

Journal: Redox Biology

Article Title: 8-oxo-7,8-dihydroguanosine (8-oxo-Guo) drives pulmonary inflammatory pathways through pattern recognition receptors

doi: 10.1016/j.redox.2026.104211

Figure Lengend Snippet: 8-oxo-Guo induces inflammatory response and promotes the M1 polarization of AMs in mice. (A) Plasma levels of TNF-α, MIP-2, and MCP-1 in mice 4 h after an injection of 8-oxo-Guo or Guo compared with the control group. ∗∗ p < 0.01, ∗∗∗ p < 0.001. (B, C) Percentages of CD86 + CD206 - cells among CD45 + F4/80 + CD11c + AMs in bronchoalveolar lavage fluid from mice that were injected with 8-oxo-Guo compared with the control group. ∗ p < 0.05.

Article Snippet: The concentrations of CXCL1, CCL2, CCL3, CCL4, CCL5, GM-CSF, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p40, IL-12p70, TNF-α, and IFN-γ in the supernatants were measured using the ABplex Mouse Cytokine 15-Plex Assay Kit (catalog no. RK05203, Abclonal, Wuhan, China).

Techniques: Clinical Proteomics, Injection, Control

Journal: Redox Biology

Article Title: 8-oxo-7,8-dihydroguanosine (8-oxo-Guo) drives pulmonary inflammatory pathways through pattern recognition receptors

doi: 10.1016/j.redox.2026.104211

Figure Lengend Snippet: 8-oxo-Guo and polyU synergistically enhanced inflammatory response in MH-S cells. (A) Concentrations of TNF-α, MIP-2, IL-1β, and MCP-1 in the culture supernatant of MH-S cells following stimulation with 1 mM 8-oxo-Guo and 20 μg/ml polyU. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. (B) Viability of MH-S cells after 24-h stimulation with 1 mM 8-oxo-Guo and 20 μg/ml polyU. (C) Concentrations of TNF-α, MIP-2, IL-1β, and MCP-1 in the culture supernatant of MLE-12 cells after 4-h co-stimulation with 1 mM 8-oxo-Guo and 20 μg/ml polyU. ∗ p < 0.05. (D) Viability of MLE-12 cells after 24-h stimulation with 1 mM 8-oxo-Guo and 20 μg/ml polyU. (E-G) Transcriptional changes in MH-S cells 2 h after stimulation with 8-oxo-Guo alone or combined with polyU. (E) Heatmap of the top 50 significantly upregulated genes following co-stimulation. (F) Bar graph of GO enrichment analysis for the top 50 upregulated genes. (G) GSEA of DEGs after co-stimulation. (H) Relative mRNA expression levels of TNF-α, MIP-2, IL-1β, and MCP-1 in cells after stimulation. ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The concentrations of CXCL1, CCL2, CCL3, CCL4, CCL5, GM-CSF, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p40, IL-12p70, TNF-α, and IFN-γ in the supernatants were measured using the ABplex Mouse Cytokine 15-Plex Assay Kit (catalog no. RK05203, Abclonal, Wuhan, China).

Techniques: Expressing

Journal: Redox Biology

Article Title: 8-oxo-7,8-dihydroguanosine (8-oxo-Guo) drives pulmonary inflammatory pathways through pattern recognition receptors

doi: 10.1016/j.redox.2026.104211

Figure Lengend Snippet: Dose-dependent suppression of 8-oxo-Guo/polyU-induced inflammation upon NOD2, TLR2, and NLRP3 inhibition. (A-F) Protein expression changes in NLR, TLR, and downstream signaling pathways (left panels) and concentrations of TNF-α, MIP-2, IL-1β, and MCP-1 in the culture supernatant (right panels) of MH-S cells following 4-h co-stimulation with 8-oxo-Guo and polyU in the presence of GSK717 (A, B) , Cu-CPT22 (C, D) , or MCC950 (E, F) . ∗∗ p < 0.01, ∗∗∗ p < 0.001. (G, H) Protein expression profiles of relevant signaling pathways (G) and cytokine concentrations in the culture supernatant (H) of MH-S cells that were treated for 4 h with 8-oxo-Guo and polyU combined with GSK717, Cu-CPT22, or MCC950, either individually or combined. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The concentrations of CXCL1, CCL2, CCL3, CCL4, CCL5, GM-CSF, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p40, IL-12p70, TNF-α, and IFN-γ in the supernatants were measured using the ABplex Mouse Cytokine 15-Plex Assay Kit (catalog no. RK05203, Abclonal, Wuhan, China).

Techniques: Inhibition, Expressing, Protein-Protein interactions

Journal: Redox Biology

Article Title: 8-oxo-7,8-dihydroguanosine (8-oxo-Guo) drives pulmonary inflammatory pathways through pattern recognition receptors

doi: 10.1016/j.redox.2026.104211

Figure Lengend Snippet: MicroRNAs that contain three consecutive uridines exert synergistic effects with 8-oxo-Guo to enhance inflammation. (A, B) Protein expression changes in NLR, TLR, and downstream signaling pathways (A) and concentrations of TNF-α, MIP-2, IL-1β, and MCP-1 in culture supernatant (B) of MH-S cells after 4-h co-stimulation with 8-oxo-Guo and microRNAs that contained varying numbers of uridine residues. ∗ p < 0.05, ∗∗∗ p < 0.001. (C, D) Protein expression changes in relevant signaling pathways (C) and cytokine concentrations in the culture supernatant (D) of MH-S cells following 4-h stimulation with triple-uridine-containing mmu-miR-23a-3p or mmu-miR-10a-5p, either alone or combined with 8-oxo-Guo. ∗∗∗ p < 0.001. (E, F) Heatmap of changes in plasma cytokine levels in mice after an injection with 8-oxo-Guo and miR-23a-3p. (G) Schematic diagram of the procedure for detecting inflammatory cytokines in mouse lung tissue. (H) Changes in inflammation-related cytokine levels in lung tissues in mice that were injected with 8-oxo-Guo and miR-23a-3p. (I) Molecular docking models of 8-oxo-Guo with NOD2, NOD2 with miR-23a-3p, and NOD2-8-oxo–Guo complex with miR-23a-3p.

Article Snippet: The concentrations of CXCL1, CCL2, CCL3, CCL4, CCL5, GM-CSF, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p40, IL-12p70, TNF-α, and IFN-γ in the supernatants were measured using the ABplex Mouse Cytokine 15-Plex Assay Kit (catalog no. RK05203, Abclonal, Wuhan, China).

Techniques: Expressing, Protein-Protein interactions, Clinical Proteomics, Injection